The TBP - TFIIA Interaction in the Response to Acidic Activators in Vivo Laurie
نویسندگان
چکیده
10306 (1982); J. K. Lanyi, Physiol. Rev. 70, 319 (1990). 3. D. Oesterhelt and J. Tittor, Trends Biochem. Sci. 14, 57 (1989); E. Bamberg, J. Bioenerg. Biomembr. 24, 181(1992). 4. R. Henderson et al., J. Mol. Biol. 213, 899 (1990). 5. M. H. J. Koch et al., EMBO J. 10, 521 (1991); M. Nakasako, M. Kataoka, Y. Amemiya, F. Tokunaga, FEBS Lett. 292, 73(1991); S. Subramaniam, M. Gerstein, D. Oesterhelt, R. Henderson, EMBO J. 12, 1 (1993). 6. M. Kataoka et al., J. Mol. Biol. 243, 621 (1994). 7. A. Blanck and D. Oesterhelt, EMBO J. 6, 265 (1987); J. K. Lanyi, A. Duschl, G. W. Hatfield, K. M. May, D. Oesterhelt, J. Biol. Chem. 265, 1253 (1990); J. Otomo, H. Tomioka, H. Sasabe, Biochim. Biophys. Acta 1112, 7 (1992); J. Soppa, J. Duschl, D. Oesterhelt, J. Bacteriol. 175, 2720 (1993). 8. T. Mogi, L. J. Stern, T. Marti, B. H. Chao, H. G. Khorana, Proc. Natl. Acad. Sci. U.S.A. 85, 4148 (1988); S. Subramaniam, T. Marti, H. G. Khorana, ibid. 87, 1013(1990). 9. Abbreviations are as follows: D, aspartate; T, threonine; and N, asparagine. Mutants are denoted with the wild-type residue, the residue number, and the new residue (for example, D85T). 10. J. Tittor, U. Schweiger, D. Oesterhelt, E. Bamberg, Biophys. J. 67, 1682 (1994). 11. J. L. Spudich, Cell 79, 747 (1994). 12. A. Maeda, T. Ogurusu, T. Yoshizawa, T. Kitagawa, Biochemistry 24, 2517 (1985); B. Schobert and J. K. Lanyi, ibid. 25,4163(1986); C. Pande, J. K. Lanyi, R. H. Callender, Biophys. J. 55, 425 (1989). 13. A. Der, R. Toth-Boconadi, L. Keszthelyi, FEBS Lett. 259, 24 (1989); L. Keszthelyi, S. Szaraz, A. Der, W. Stoeckenius, Biochim. Biophys. Acta 1018, 260 (1990); A. Deretal., Proc. Natl. Acad. Sci. U.S.A. 88, 4751 (1991). 14. T. Marti, S. J. Rbsselet, H. Otto, M. P. Heyn, H. G. Khorana, J. Biol. Chem. 266,18674 (1991); T. Marti, H. Otto, S. J. Rbsselet, M. P. Heyn, H. G. Khorana, ibid. 267, 16922 (1992). 15. L. S. Brown and J. K. Lanyi, unpublished results. 16. J. K. Lanyi and D. Oesterhelt, J. Biol. Chem. 257, 2674 (1982). 17. The D85T gene was expressed in H. salinarium Pho81 cells with a plasmid vector that contains a gene for novobiocin resistance. Envelope vesicles were prepared as described [J. K. Lanyi and R. E. MacDonald, Methods Enzymol. 56, 398 (1979)]. 18. S. A. Sundberg et al., J. Bacteriol. 172, 2328 (1990). 19. A. Duschl, J. K. Lanyi, L. Zimanyi, J. Biol. Chem. 265, 1261 (1990). 20. A. Matsuno-Yagi and Y. Mukohata, Biochem. Biophys. Res. Commun. 78, 237 (1977). 21. B. Scharf and M. Engelhard, Biochemistry 33, 6387 (1994). 22. T. Ogurusu, A. Maeda, T. Yoshizawa, J. Biochem. 95, 1073 (1984); B. Schobert, J. K. Lanyi, D. Oesterhelt, J. Biol. Chem. 261, 2690 (1986). 23. G. Varo et al., in preparation. 24. J. Sasaki, unpublished results. 25. J. Tittor, D. Oesterhelt, R. Maurer, H. Desel, R. Uhl, Biophys. J. 52, 999 (1987); J. K. Lanyi and V. Vodyanoy, Biochemistry 25, 1465 (1986). 26. G. Varo et al., Biophys. J. 68, 2062 (1995). 27. T. Kouyama, A. Nasuda-Kouyama, A. Ikegami, M. K. Mathew, W. Stoeckenius, Biochemistry 27, 5855 (1988); H. Otto et al., Proc. Natl. Acad. Sci. U.S.A. 86, 9228 (1989); L. Zimanyi, Y. Cao, R. Needleman, M. Ottolenghi, J. K. Lanyi, Biochemistry 32, 7669 (1993). 28. L. Zimanyi, L. Keszthelyi, J. K. Lanyi, Biochemistry 28, 5165 (1989). 29. We are grateful to J. Spudich for the Pho81 strain. Funded by the U.S. Department of Energy (grant DEFG03-86ER13525 to J.K.L. and grant DEFG0292ER20089 to R.N.), NIH (grant GM 29498 to J.K.L.), and NSF (grant MCB-9202209 to R.N.). J.S. acknowledges a Grant-in-Aid for International Research (06044123) from the Japanese Ministry of Education, Culture and Science that funded his stay in Irvine, CA.
منابع مشابه
The TBP-TFIIA interaction in the response to acidic activators in vivo.
A yeast TBP mutant (N2-1) is described here that is defective specifically in responding to acidic activators in vivo. N2-1 does not support activation by Gal4, Ace1, and Gcn4, but appears unaffected for constitutive transcription, repression by the Cyc8-Tup1 and Not complexes, and transcription by polymerase I (Pol) and Pol III. In vitro, N2-1 fails to interact with TFIIA, but it associates no...
متن کاملA new class of activation-defective TATA-binding protein mutants: evidence for two steps of transcriptional activation in vivo.
Using a genetic screen, we isolated four TATA-binding protein (TBP) mutants that are specifically defective in vivo for the response to acidic activators. In contrast to previously described activation-defective TBP mutants, these TBP derivatives are not specifically defective for interactions with TATA elements or TFIIA. Three of these derivatives interact normally with a TATA element, TFIIA, ...
متن کاملA severely defective TATA-binding protein-TFIIB interaction does not preclude transcriptional activation in vivo.
In yeast cells, mutations in the TATA-binding protein (TBP) that disrupt the interaction with the TATA element or with TFIIA can selectively impair the response to acidic activator proteins. We analyzed the transcriptional properties of TBP derivatives in which residues that directly interact with TFIIB were replaced by alanines. Surprisingly, a derivative with a 50-fold defect in TBP-TFIIB-TAT...
متن کاملTFIIA has activator-dependent and core promoter functions in vivo.
The physiological role of TFIIA was investigated by analyzing transcription in a yeast strain that contains a TATA-binding protein (TBP) mutant (N2-1) defective for interacting with TFIIA. In cells containing N2-1, transcription from a set of artificial his3 promoters dependent on different activators is generally reduced by a similar extent, indicating that TFIIA function is largely nonselecti...
متن کاملAnalysis of TFIIA function In vivo: evidence for a role in TATA-binding protein recruitment and gene-specific activation.
Activation of transcription can occur by the facilitated recruitment of TFIID to promoters by gene-specific activators. To investigate the role of TFIIA in TFIID recruitment in vivo, we exploited a class of yeast TATA-binding protein (TBP) mutants that is activation and DNA binding defective. We found that co-overexpression of TOA1 and TOA2, the genes that encode yeast TFIIA, overcomes the acti...
متن کاملMutations on the DNA-binding surface of TATA-binding protein can specifically impair the response to acidic activators in vivo.
The TATA-binding protein (TBP) contains a concave surface that interacts specifically with TATA promoter elements and a convex surface that mediates protein-protein interactions with general and gene-specific transcription factors. Biochemical experiments suggest that interactions between activator proteins and TBP are important in stimulating transcription by the RNA polymerase II machinery. T...
متن کامل